US 10,072,104 B2
Polymer backbone element tagsGeneral
US 10,072,104 B2
Polymer backbone element tags
Tech Center:
1600
Examiner:
Shafiqul Haq
Art Unit:
1677
Inventors:
MITCHELL A. WINNIK; MARK NITZ; VLADIMIR BARANOV; XUDONG LOU
Assignee:
STANDARD BIOTOOLS CANADA INC.
Priority:
05/27/06
Filed:
10/05/15
Granted:
09/11/18
Expiration:
05/27/26
Abstract
Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).
Cooperative Patent Classification (CPC)
G01G01N33/57426G01NC08F220/36C08FY10T436/24Y10T