OVEREXPRESSION OF N-GLYCOSYLATION PATHWAY REGULATORS TO MODULATE GLYCOSYLATION OF RECOMBINANT PROTEINS

Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the overexpression of proteins involved in the N-glycosylation pathway.

Overexpression of N-glycosylation pathway regulators to modulate glycosylation of recombinant proteins

BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING

Glycosyltransferases (2.4)-Hexosyltransferases (2.4.1)-Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.101)

ENZYMES

Preparation of peptides or proteins -Glycopeptides, glycoproteins

FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE 

Glycosyltransferases (2.4)-Hexosyltransferases (2.4.1)-Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.143)

ORGANIC CHEMISTRY

Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies 

PEPTIDES 

Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes -Transferases (2.) -Glycosyltransferases (2.4)-Hexosyltransferases (2.4.1)

MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA 

Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor -Recombinant DNA-technology-Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression-Vectors or expression systems specially adapted for eukaryotic hosts-for animal cells

Immunoglobulins specific features-characterized by post-translational modification-Glycosylation, sialylation, or fucosylation

Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof-from animals; from humans-Receptors; Cell surface antigens; Cell surface determinants 

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