DECREASING ORNITHINE PRODUCTION TO DECREASE HIGH MANNOSE GLYCOFORM CONTENT OF RECOMBINANT PROTEINS

The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by regulating ornithine metabolism during cell culture.

Decreasing ornithine production to decrease high mannose glycoform content of recombinant proteins

BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING

Specific components of cell culture medium-Organic components-Amino acids

MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA 

Specific components of cell culture medium-Organic components-Amines, e.g. putrescine

ORGANIC CHEMISTRY

Immunoglobulins specific features-characterized by their source of isolation or production-Specific host cells or culture conditions, e.g. components, pH or temperature

PEPTIDES 

Immunoglobulins specific features-characterized by post-translational modification-Glycosylation, sialylation, or fucosylation

Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof-from animals; from humans-Cytokines; Lymphokines; Interferons

Preparation of peptides or proteins -Glycopeptides, glycoproteins

FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE 

Genetically modified cells-Cells for production

Culture process characterised by the use of hydrostatic pressure, flow or shear forces

Specific components of cell culture medium-Organic components-Amino acids-other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine

Microorganisms, e.g. protozoa; Compositions thereof ; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor-Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound 

Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies 

Specific components of cell culture medium-Serum-free medium, which may still contain naturally-sourced components

Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor; -Animal cells or tissues; Human cells or tissues -Vertebrate cells-Cells of the genital tract; Non-germinal cells from gonads-Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells 

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