US 10,894,972 B2
METHODS FOR INCREASING MANNOSE CONTENT OF RECOMBINANT PROTEINSGeneral
US 10,894,972 B2
METHODS FOR INCREASING MANNOSE CONTENT OF RECOMBINANT PROTEINS
Tech Center:
1600 Biotechnology and Organic Chemistry
Examiner:
Paul J Holland
Art Unit:
1656 Fermentation, Microbiology, Isolated and Recombinant Proteins/Enzyme
Agent:
Henry P. Wu
Inventors:
Chung-Jr HUANG; Xiaoming YANG
Assignee:
Priority:
05/29/14
Filed:
08/09/19
Granted:
01/19/21
Expiration:
05/30/33
Abstract
The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation.
Cooperative Patent Classification (CPC)
C12C12P21/005C12N2500/34C12PC07K2317/41C07K