US 11,098,079 B2
Charged Depth Filtration of Antigen-Binding ProteinsGeneral
US 11,098,079 B2
Charged Depth Filtration of Antigen-Binding Proteins
Tech Center:
1600 Biotechnology and Organic Chemistry
Examiner:
Daniel E Kolker
Art Unit:
1644 Immunology, Receptor/Ligands, Cytokines Recombinant Hormones, and Molecular Biology
Inventors:
Hai Hoang; Rafael Gonzalez; Junfen Ma
Assignee:
Priority:
08/12/16
Filed:
02/08/18
Granted:
08/24/21
Expiration:
07/21/36
Abstract
Methods of producing an aqueous formulation of an antigen-binding protein or enhancing re-oxidation of an antigen-binding protein are disclosed. The methods comprise (a) contacting an aqueous solution comprising antigen-binding protein molecules with a charged depth filter under conditions sufficient to enhance re-oxidation of the antigen-binding protein molecules and achieve a decrease in the percentage of reduced antigen-binding protein molecules, compared to the percentage of reduced antigen-binding protein molecules observed prior to step (a); and (b) optionally, measuring the amount or relative amount of reduced antigen-binding protein molecules. Formulations comprising a re-oxidized antigen-binding protein are also described.
Cooperative Patent Classification (CPC)
C07C07K2317/40C07KG01N27/44791G01NB01D15/3809B01D