US 8,168,381 B2
Template directed split and mix systhesis of small molecule librariesGeneral
US 8,168,381 B2
Template directed split and mix systhesis of small molecule libraries
Tech Center:
1600 Biotechnology and Organic Chemistry
Examiner:
Jezia Riley
Art Unit:
1637 Molecular Biology, Bioinformatics, Nucleic Acids, Recombinant DNA and RNA, Gene Regulation, Nucleic Acid Amplification, Animals and Plants, Combinatorial/ Computational Chemistry
Inventors:
Peter Birk Rasmussen
Assignee:
CHEMGENE APS
Priority:
11/22/04
Filed:
11/22/05
Granted:
05/01/12
Expiration:
06/03/24
Abstract
The present invention provides a method for combining the advantages of encoded molecule fragments made by split and mix synthesis with the advantages of template directed synthesis of molecules. The method provided in the invention comprises the steps of: Adding a linker molecule L to one or more reaction wells; Adding a molecule fragment to each of said reaction wells; Adding an oligonucleotide identifier to each of said reaction wells; Subjecting said wells to conditions sufficient to allow said molecule fragments and said oligonucleotie identifiers to become attached to said linker molecule, or conditions sufficient for said molecule fragments to bind to other molecule fragments and sufficient for said oligonucleotide identifiers to bind to other oligonucleotide identifiers; Combining the contents of said one or more reaction wells; Optionally, distributing the combined product to one or more new reaction wells; Optionally, repeating steps b) to e) one or more times; Contacting the resulting bifunctional molecule(s) of step e) or g) with one or more Contacting the resulting bifunctional molecule(s) of step e) or g) with one or more (oligonucleotide) templates each capable of hybridizing to at least one of the oligonucleotide identifiers added in step c).
Cooperative Patent Classification (CPC)
B01B01J19/0046B01JC12N15/1068C12N