US 9,011,872 B2
PCV2 immunogenic compositions and methods of producing such compositionsGeneral
US 9,011,872 B2
PCV2 immunogenic compositions and methods of producing such compositions
Tech Center:
1600 Biotechnology and Organic Chemistry
Examiner:
Shanon A Foley
Art Unit:
1648 Immunology, Receptor/Ligands, Cytokines Recombinant Hormones, and Molecular Biology
Inventors:
Marc Allan Eichmeyer; Merrill Lynn Schaeffer; Gregory Paul Nitzel
Assignee:
Priority:
12/30/04
Filed:
12/27/12
Granted:
04/21/15
Expiration:
12/30/24
Abstract
An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time-consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
Cooperative Patent Classification (CPC)
A61A61K2039/55505A61P31/12A61KC12N2750/10022C12NC07K14/005C07K