US 9,695,468 B2
Methods for droplet-based sample preparationGeneral
US 9,695,468 B2
Methods for droplet-based sample preparation
Tech Center:
1600 Biotechnology and Organic Chemistry
Examiner:
Narayan Bhat
Art Unit:
1634 Molecular Biology, Bioinformatics, Nucleic Acids, Recombinant DNA and RNA, Gene Regulation, Nucleic Acid Amplification, Animals and Plants, Combinatorial/ Computational Chemistry
Inventors:
Benjamin Hindson; Serge Saxonov; Michael Schnall-Levin
Assignee:
Priority:
08/14/12
Filed:
02/17/15
Granted:
07/04/17
Expiration:
08/14/32
Abstract
This disclosure provides microwell capsule array devices. The microwell capsule array devices are generally capable of performing one or more sample preparation operations. Such sample preparation operations may be used as a prelude to one more or more analysis operations. For example, a device of this disclosure can achieve physical partitioning and discrete mixing of samples with unique molecular identifiers within a single unit in preparation for various analysis operations. The device may be useful in a variety of applications and most notably nucleic-acid-based sequencing, detection and quantification of gene expression and single-cell analysis.
Cooperative Patent Classification (CPC)
B01B01L2200/0647B01J19/0046B01LC12Q2563/159C12N15/1065C12Q