US 9,708,358 B2
Massive parallel method for decoding DNA and RNAGeneral
US 9,708,358 B2
Massive parallel method for decoding DNA and RNA
Tech Center:
1600
Examiner:
Layla Berry
Art Unit:
1673
Agent:
Inventors:
Zengmin Li; Yasuhiro Itagaki; Jingyue Ju; John Robert Edwards
Priority:
10/06/00
Filed:
12/15/16
Granted:
07/18/17
Expiration:
08/25/25
Abstract
This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analog after the nucleotide analog is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogs which comprise unique labels attached to the nucleotide analog through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
Cooperative Patent Classification (CPC)
C12C12Q2525/186C12QC07H19/14C07B2200/11C07HC40B40/00C40B