US 9,856,287 B2
Refolding proteins using a chemically controlled redox state
Log In
Please sign up or log in to access the advanced features of
Ex Parte Enterprise.
or Log in

General

US 9,856,287 B2
Refolding proteins using a chemically controlled redox state
Tech Center:
1600 Biotechnology and Organic Chemistry
Examiner:
Yunsoo Kim
Art Unit:
1644 Immunology, Receptor/Ligands, Cytokines Recombinant Hormones, and Molecular Biology
Agent:
Ropes & Gray LLP
Inventors:
Joseph Edward SHULTZ; Roger HART; Ronald Nixon KEENER
Assignee:
AMGEN INC.
Priority:
06/22/09
Filed:
02/01/17
Granted:
01/02/18
Expiration:
06/22/29
Abstract
A method of refolding proteins expressed in non-mammalian cells present in concentrations of 2.0 g/L or higher is disclosed. The method comprises identifying the thiol pair ratio and the redox buffer strength to achieve conditions under which efficient folding at concentrations of 2.0 g/L or higher is achieved and can be employed over a range of volumes, including commercial scale.
Cooperative Patent Classification (CPC)
C07C07K2319/30C07K

Analytics

Cases

Patent Assignments

Citations