US 9,868,985 B2 | Massive parallel method for decoding DNA and RNA

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US 9,868,985 B2

Massive parallel method for decoding DNA and RNA

Tech Center:

1600 Biotechnology and Organic Chemistry

Examiner:

Jezia Riley

Art Unit:

1637 Molecular Biology, Bioinformatics, Nucleic Acids, Recombinant DNA and RNA, Gene Regulation, Nucleic Acid Amplification, Animals and Plants, Combinatorial/ Computational Chemistry

Agent:

Cooper & Dunham LLP

Inventors:

Zengmin Li; Yasuhiro Itagaki; Jingyue Ju; John Robert Edwards

Assignee:

THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

Priority:

10/06/00

Filed:

07/12/17

Granted:

01/16/18

Expiration:

10/06/20

Abstract

This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analog after the nucleotide analog is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogs which comprise unique labels attached to the nucleotide analog through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

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