US RE46,817 E1
Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophoresGeneral
US RE46,817 E1
Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
Tech Center:
3900 Reexam/Abandonments
Examiner:
Alan Diamond
Art Unit:
3991 Central Reexamination Unit
Inventors:
Brent S. Gaylord; Guillermo C. Bazan; Shu Wang
Priority:
08/26/02
Filed:
03/29/17
Granted:
05/01/18
Expiration:
03/29/37
Abstract
Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor polynucleotide complementary to the target polynucleotide. The sensor polynucleotide comprises a signaling chromophore to receive energy from the excited multichromophore and increase emission in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.